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cd163 protein  (MedChemExpress)


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    Structured Review

    MedChemExpress cd163 protein
    Increased macrophages and elevated <t>CD163</t> expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.
    Cd163 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd163+protein/pmc12319426-55-0-5?v=MedChemExpress
    Average 93 stars, based on 4 article reviews
    cd163 protein - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome"

    Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

    Journal: Journal of Inflammation Research

    doi: 10.2147/JIR.S532920

    Increased macrophages and elevated CD163 expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.
    Figure Legend Snippet: Increased macrophages and elevated CD163 expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.

    Techniques Used: Expressing, Immunofluorescence, Control

    Increased M1 and M2 macrophages and elevated CD163 expression in M1 and M2 macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the ovaries of control and PCOS mice (scale bar = 50 μm). ( b ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the uterus of control and PCOS mice (scale bar = 50 μm). ( c ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the ovaries. ( d ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the uterus. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Increased M1 and M2 macrophages and elevated CD163 expression in M1 and M2 macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the ovaries of control and PCOS mice (scale bar = 50 μm). ( b ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the uterus of control and PCOS mice (scale bar = 50 μm). ( c ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the ovaries. ( d ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the uterus. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Immunofluorescence, Control

    Increased apoptosis in CD163 + ovarian cells from PCOS mice. ( a ) Representative images of TUNEL and CD163 in the ovaries of control and PCOS mice via immunofluorescence analysis (scale bar = 50 μm). ( b ) Quantification of TUNEL + and CD163 + TUNEL + cell proportions in the ovaries. ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Increased apoptosis in CD163 + ovarian cells from PCOS mice. ( a ) Representative images of TUNEL and CD163 in the ovaries of control and PCOS mice via immunofluorescence analysis (scale bar = 50 μm). ( b ) Quantification of TUNEL + and CD163 + TUNEL + cell proportions in the ovaries. ** p < 0.01, *** p < 0.001.

    Techniques Used: TUNEL Assay, Control, Immunofluorescence



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    Increased macrophages and elevated <t>CD163</t> expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.
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    Increased macrophages and elevated <t>CD163</t> expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.
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    Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating <t>CD163-positive</t> cells, green arrows showing CD68-positive cells, and yellow arrows marking <t>CD163</t> and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.
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    Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating <t>CD163-positive</t> cells, green arrows showing CD68-positive cells, and yellow arrows marking <t>CD163</t> and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.
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    Image Search Results


    Increased macrophages and elevated CD163 expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.

    Journal: Journal of Inflammation Research

    Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

    doi: 10.2147/JIR.S532920

    Figure Lengend Snippet: Increased macrophages and elevated CD163 expression in macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163 and F4/80 in ovaries and uteri of control and PCOS mice (scale bar = 100 μm). ( b ) Quantification of CD163 + , F4/80 + , and F4/80 + CD163 + cell proportions in the ovaries and uteri of control and PCOS mice. * p < 0.05, ** p < 0.01, ns: p > 0.05.

    Article Snippet: CD163 protein was acquired from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Immunofluorescence, Control

    Increased M1 and M2 macrophages and elevated CD163 expression in M1 and M2 macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the ovaries of control and PCOS mice (scale bar = 50 μm). ( b ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the uterus of control and PCOS mice (scale bar = 50 μm). ( c ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the ovaries. ( d ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the uterus. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Journal of Inflammation Research

    Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

    doi: 10.2147/JIR.S532920

    Figure Lengend Snippet: Increased M1 and M2 macrophages and elevated CD163 expression in M1 and M2 macrophages in the ovary and uterus of PCOS mice. ( a ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the ovaries of control and PCOS mice (scale bar = 50 μm). ( b ) Representative immunofluorescence images of CD163, F4/80, iNOS, and Arg1 in the uterus of control and PCOS mice (scale bar = 50 μm). ( c ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the ovaries. ( d ) Quantification of F4/80 + iNOS + CD163 + and F4/80 + Arg1 + CD163 + cell proportions in the uterus. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: CD163 protein was acquired from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Immunofluorescence, Control

    Increased apoptosis in CD163 + ovarian cells from PCOS mice. ( a ) Representative images of TUNEL and CD163 in the ovaries of control and PCOS mice via immunofluorescence analysis (scale bar = 50 μm). ( b ) Quantification of TUNEL + and CD163 + TUNEL + cell proportions in the ovaries. ** p < 0.01, *** p < 0.001.

    Journal: Journal of Inflammation Research

    Article Title: Increased CD163+ Macrophage Activation and High Expression of CD163 Promote Granulosa Cell Apoptosis in Polycystic Ovary Syndrome

    doi: 10.2147/JIR.S532920

    Figure Lengend Snippet: Increased apoptosis in CD163 + ovarian cells from PCOS mice. ( a ) Representative images of TUNEL and CD163 in the ovaries of control and PCOS mice via immunofluorescence analysis (scale bar = 50 μm). ( b ) Quantification of TUNEL + and CD163 + TUNEL + cell proportions in the ovaries. ** p < 0.01, *** p < 0.001.

    Article Snippet: CD163 protein was acquired from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: TUNEL Assay, Control, Immunofluorescence

    Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.

    Journal: Frontiers in Nutrition

    Article Title: High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways

    doi: 10.3389/fnut.2025.1562509

    Figure Lengend Snippet: Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.

    Article Snippet: BCA protein quantitation kit (Boster Bio, Cat # AR0146), RIPA lysis buffer (Boster Bio, Cat # 0105), protease inhibitor cocktails (Boster, Bio Cat # AR1182), phosphatase inhibitor (Boster Bio, Cat # AR1183), color pre-dyed protein marker (Boster Bio, Cat # AR1113), Western-specific primary and secondary antibody diluent (Boster Bio, Cat # AR1017), wash buffer TBS-T (Boster Bio, Cat # AR0195-10), ECL chemiluminescent reagent (Boster Bio, Cat # AR1196), BSA TBS buffer system blocking solution (Boster Bio, Cat # AR0189), NF-κB antibody (Boster Bio, Cat # A01228-1), GAPDH antibody (Boster Bio, Cat # M00227), HRP-conjugated goat anti-rabbit IgG (Boster Bio, Cat # BA1054), CD163 antibody (Boster Bio, Cat # A00812-2), CD11c antibody (Boster Bio, Cat # A00357-3), CD68 antibody (Boster Bio, Cat # BA3638), fluorescent (DyLight 488) labeled goat anti-rabbit IgG (Boster Bio, Cat # BA1127), fluorescent (DyLight 594) labeled goat anti-rabbit IgG (Boster Bio, Cat # BA1142), rat TNF-α ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0180R1), rat IL-1β ELISA kit (Boster Bio, Cat # EK0393), rat IL-4 ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0191R1), rat IL-10 ELISA kit (Boster Bio, Cat # EK0418), 25 VD3 assay kit (Roche Diagnostics, Cat # 07028148190), TAOC assay kit (Nanjing Jiancheng, Cat # A015-3-1), MDA assay kit (Nanjing Jiancheng, Cat # A003-1-2), and free fatty acid kit (Kunchuang Biotechnology, Xian, China, Cat # SK125-2).

    Techniques: Double Staining, Expressing